DdeI Polymorphism in Coding Region of Goat POU1F1 Gene and Its Association with Production Traits
نویسندگان
چکیده
POU1F1 is a positive regulator for GH, PRL and TSHβ and its mutations associate with production traits in ruminant animals. We described a DdeI PCR-RFLP method for detecting a silent allele in the goat POU1F1 gene: TCT (241Ser)>TCG (241Ser). Frequencies of D1 allele varied from 0.600 to 1.000 in Chinese 801 goats. Significant associations of DdeI polymorphism with production traits were found in milk yield (*p<0.05), litter size (*p<0.05) and one-year-old weight (*p<0.05) between different genotypes. Individuals with genotype D1D1 had a superior performances when compared to those with genotype D1D2 (*p<0.05). Hence, the POU1F1 gene was suggested to the potential candidate gene for superior milk performance, reproduction trait and weight trait. Genotype D1D1, characterized by a DdeI PCR-RFLP detection, was recommended to geneticists and breeders as a molecular marker for better performance in the goat industry. (
منابع مشابه
Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
The purpose of the study was to detect the AluI and DdeI polymorphisms within POU1F1 gene exon 6 and 3'UTR region in Turkish sheep breeds, and their association with milk productive traits. Jugular blood samples were collected from 50 Sakiz, 50 White Karaman, and 50 Awassi sheep using EDTA as an anticoagulant. PCR-RFLP and sequencing analysis were performed to investigate possible polymorphisms...
متن کاملA PCR-RFLP investigation on PROP1 gene polymorphism and its association with milk production and growth traits in Mahabadi goats
Prophet of POU1F1 (PROP1) plays direct or indirect important roles in the morphogenesis of pituitary gonadotropes as well as lactotropes, somatotropes and caudomedial thyrotropes. It also controls the expression of growth hormone, prolactin and thyrotropin subunits through regulatory PIT1 factor. These hormones have important influences on production traits in most animal species including the ...
متن کاملPolymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits.
The purpose of the study was to detect the AluI and DdeI polymorphisms within POU1F1 gene exon 6 and 3'UTR region in Turkish sheep breeds, and their association with milk productive traits. Jugular blood samples were collected from 50 Sakiz, 50 White Karaman, and 50 Awassi sheep using EDTA as an anticoagulant. PCR-RFLP and sequencing analysis were performed to investigate possible polymorphisms...
متن کاملارتباط چند شکلی ژن POU1F1 با صفات رشد و شیر در بز مهابادی
Abstract One of the latest methods which can improve the accuracy of prediction and response to selection is the selection based on markers. Research has shown that the POU1F1 (Pit-1) gene has important role in the regulation of animal growth and associated with carcass, weight and milk traits. The purpose of this research was to study polymorphisms of POU1F1 gene and their association with...
متن کاملPolymorphism of the Growth Hormone Gene and Its Effect on Production and Reproduction Traits in Goat
Growth hormone (GH) has an effect on a broad variety of physiological parameters such as lactation, reproduction, growth and metabolism. Thus, the aim of this study was to detect GH gene polymorphism and its association with breeding values of production and reproduction traits in Raini Cashmere goat. Breeding values were estimated using records on 26731 Raini Cashmere goats. To study GH gene p...
متن کامل